Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 4025..4619 Replicon plasmid 4
Accession NZ_LR890526
Organism Burkholderia cepacia isolate MINF_4A-sc-2280433

Toxin (Protein)


Gene name PumA Uniprot ID A0A228JB64
Locus tag ISX57_RS35470 Protein ID WP_027811055.1
Coordinates 4326..4619 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A228JAU5
Locus tag ISX57_RS35465 Protein ID WP_027811056.1
Coordinates 4025..4324 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISX57_RS35450 1382..1783 - 402 WP_027811058.1 hypothetical protein -
ISX57_RS35455 2800..3303 + 504 WP_143277545.1 hypothetical protein -
ISX57_RS35460 3551..3862 + 312 WP_027811057.1 hypothetical protein -
ISX57_RS35465 4025..4324 - 300 WP_027811056.1 putative addiction module antidote protein Antitoxin
ISX57_RS35470 4326..4619 - 294 WP_027811055.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISX57_RS35475 4724..4867 - 144 WP_154233621.1 hypothetical protein -
ISX57_RS35480 4924..5145 - 222 WP_027811054.1 DUF3717 domain-containing protein -
ISX57_RS35485 5190..5741 - 552 WP_077186813.1 hypothetical protein -
ISX57_RS35490 5716..5982 - 267 WP_027811052.1 hypothetical protein -
ISX57_RS35495 7026..9155 - 2130 WP_039350079.1 ParB/RepB/Spo0J family partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..114206 114206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(11-87)

Antitoxin

Uncharacterized protein HI1420

(5-91)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10854.50 Da        Isoelectric Point: 10.6086

>T291280 WP_027811055.1 NZ_LR890526:c4619-4326 [Burkholderia cepacia]
MRIEQTDDFAKWLRGLRDHIARAQIAKRIQRLARGQYGDMKPVGAGVSEMRVHVGPGYRVYFVQRGSTLVVLLCGGDKST
QQRDIERAIALSAQLGD

Download         Length: 294 bp

>T291280 NZ_LR890526:c4619-4326 [Burkholderia cepacia]
ATGAGAATCGAGCAGACAGACGACTTCGCGAAATGGTTGCGCGGCCTACGCGATCACATCGCGCGCGCGCAGATCGCCAA
GCGGATTCAACGGCTCGCGCGCGGCCAATATGGGGACATGAAGCCGGTGGGTGCCGGAGTGAGCGAAATGCGCGTGCACG
TCGGCCCCGGCTATCGCGTCTACTTTGTCCAACGAGGCTCAACGCTCGTCGTCCTGCTATGCGGTGGCGACAAGTCAACG
CAGCAGCGCGACATTGAGCGTGCTATCGCGCTTTCGGCGCAATTGGGAGATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10716.01 Da        Isoelectric Point: 4.6364

>AT291280 WP_027811056.1 NZ_LR890526:c4324-4025 [Burkholderia cepacia]
MGIKTTPFDAAEYLDTPEAQAEYLRISFETGDSADIQEAIGTVARARGMTQIARDAGVGRESLYKALDEHGNPEFATILR
VVRALGLTLTVTTPVHEHA

Download         Length: 300 bp

>AT291280 NZ_LR890526:c4324-4025 [Burkholderia cepacia]
ATGGGTATCAAGACAACGCCGTTCGATGCGGCCGAATACCTCGACACGCCCGAGGCGCAGGCCGAATATCTGCGGATTTC
GTTCGAGACCGGCGATTCCGCCGACATTCAGGAAGCCATCGGCACCGTGGCGCGTGCGCGTGGCATGACGCAGATTGCGC
GCGACGCGGGCGTCGGCCGCGAAAGCCTCTACAAAGCGCTGGACGAGCACGGCAACCCGGAATTCGCGACGATCCTGCGT
GTTGTGCGCGCGCTCGGCCTCACGTTGACCGTGACGACGCCCGTACACGAGCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A228JB64

Antitoxin

Source ID Structure
AlphaFold DB A0A228JAU5

References