Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4347898..4348708 Replicon chromosome
Accession NZ_LR890500
Organism Klebsiella pneumoniae isolate KSB1_5H-sc-2280283

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag JMX76_RS21490 Protein ID WP_004178461.1
Coordinates 4348175..4348708 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JMX76_RS21485 Protein ID WP_002887278.1
Coordinates 4347898..4348164 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMX76_RS21465 4343770..4344114 - 345 WP_064162549.1 cation efflux system protein CusF -
JMX76_RS21470 4344132..4345517 - 1386 WP_064162550.1 efflux transporter outer membrane subunit -
JMX76_RS21475 4345689..4346372 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
JMX76_RS21480 4346362..4347795 + 1434 WP_064162551.1 Cu(+)/Ag(+) sensor histidine kinase -
JMX76_RS21485 4347898..4348164 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JMX76_RS21490 4348175..4348708 + 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
JMX76_RS21495 4348756..4349877 - 1122 WP_004214138.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T291208 WP_004178461.1 NZ_LR890500:4348175-4348708 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT291208 WP_002887278.1 NZ_LR890500:4347898-4348164 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References