Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 141966..142491 Replicon plasmid 2
Accession NZ_LR890467
Organism Escherichia coli isolate MSB1_7A-sc-2280394

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JMW16_RS25375 Protein ID WP_001159868.1
Coordinates 141966..142271 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JMW16_RS25380 Protein ID WP_000813634.1
Coordinates 142273..142491 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW16_RS25360 137876..139042 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
JMW16_RS25365 139630..140385 - 756 WP_000852146.1 replication initiation protein RepE -
JMW16_RS25370 141159..141965 - 807 WP_000016982.1 site-specific integrase -
JMW16_RS25375 141966..142271 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JMW16_RS25380 142273..142491 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JMW16_RS25385 143199..144194 + 996 WP_000246636.1 hypothetical protein -
JMW16_RS25390 144198..145130 + 933 WP_000991832.1 hypothetical protein -
JMW16_RS25395 145267..145964 - 698 Protein_176 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B / blaCTX-M-27 / erm(B) senB 1..160530 160530
- flank IS/Tn - - 145267..145770 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T291119 WP_001159868.1 NZ_LR890467:c142271-141966 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT291119 WP_000813634.1 NZ_LR890467:c142491-142273 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References