Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4456494..4457077 Replicon chromosome
Accession NZ_LR890466
Organism Escherichia coli isolate MSB1_7A-sc-2280394

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag JMW16_RS21185 Protein ID WP_000254738.1
Coordinates 4456742..4457077 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JMW16_RS21180 Protein ID WP_000581937.1
Coordinates 4456494..4456742 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW16_RS21170 4452833..4454134 + 1302 WP_001551563.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JMW16_RS21175 4454182..4456416 + 2235 WP_001551562.1 GTP diphosphokinase -
JMW16_RS21180 4456494..4456742 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JMW16_RS21185 4456742..4457077 + 336 WP_000254738.1 endoribonuclease MazF Toxin
JMW16_RS21190 4457149..4457940 + 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
JMW16_RS21195 4458168..4459805 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JMW16_RS21200 4459893..4461191 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T291109 WP_000254738.1 NZ_LR890466:4456742-4457077 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT291109 WP_000581937.1 NZ_LR890466:4456494-4456742 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References