Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2777773..2778270 Replicon chromosome
Accession NZ_LR890466
Organism Escherichia coli isolate MSB1_7A-sc-2280394

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JMW16_RS13365 Protein ID WP_001173343.1
Coordinates 2777773..2777946 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JMW16_RS13370 Protein ID WP_000398619.1
Coordinates 2777974..2778270 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW16_RS13355 2774443..2775900 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
JMW16_RS13360 2776137..2777654 + 1518 WP_001550472.1 lysine--tRNA ligase -
JMW16_RS13365 2777773..2777946 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JMW16_RS13370 2777974..2778270 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JMW16_RS13375 2778499..2778771 - 273 WP_000405647.1 N-acetyltransferase -
JMW16_RS13380 2778783..2779013 - 231 WP_001550470.1 (4Fe-4S)-binding protein -
JMW16_RS13385 2779193..2780824 + 1632 WP_001216477.1 sensor histidine kinase -
JMW16_RS13390 2780821..2781540 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2732480..2778270 45790


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T291101 WP_001173343.1 NZ_LR890466:c2777946-2777773 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT291101 WP_000398619.1 NZ_LR890466:c2778270-2777974 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References