Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5256055..5256680 Replicon chromosome
Accession NZ_LR890457
Organism Klebsiella pneumoniae isolate KSB1_6G-sc-2280276

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag JMX47_RS25720 Protein ID WP_004187928.1
Coordinates 5256055..5256438 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JMX47_RS25725 Protein ID WP_004150355.1
Coordinates 5256438..5256680 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMX47_RS25705 5253421..5254323 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JMX47_RS25710 5254320..5254955 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JMX47_RS25715 5254952..5255881 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JMX47_RS25720 5256055..5256438 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
JMX47_RS25725 5256438..5256680 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JMX47_RS25730 5256874..5257791 + 918 WP_004187929.1 alpha/beta hydrolase -
JMX47_RS25735 5257805..5258746 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JMX47_RS25740 5258791..5259228 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JMX47_RS25745 5259225..5260085 - 861 WP_004146232.1 virulence factor BrkB family protein -
JMX47_RS25750 5260079..5260678 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T291065 WP_004187928.1 NZ_LR890457:c5256438-5256055 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT291065 WP_004150355.1 NZ_LR890457:c5256680-5256438 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References