Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4441566..4442376 Replicon chromosome
Accession NZ_LR890456
Organism Klebsiella pneumoniae isolate INF032-sc-2279918

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag JMV68_RS21645 Protein ID WP_004178461.1
Coordinates 4441566..4442099 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JMV68_RS21650 Protein ID WP_002887278.1
Coordinates 4442110..4442376 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV68_RS21640 4440397..4441518 + 1122 WP_009309849.1 cupin domain-containing protein -
JMV68_RS21645 4441566..4442099 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
JMV68_RS21650 4442110..4442376 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JMV68_RS21655 4442479..4443912 - 1434 WP_023280821.1 Cu(+)/Ag(+) sensor histidine kinase -
JMV68_RS21660 4443902..4444585 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
JMV68_RS21665 4444758..4446143 + 1386 WP_040238382.1 efflux transporter outer membrane subunit -
JMV68_RS21670 4446161..4446505 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T291047 WP_004178461.1 NZ_LR890456:c4442099-4441566 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT291047 WP_002887278.1 NZ_LR890456:c4442376-4442110 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References