Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4676670..4677480 Replicon chromosome
Accession NZ_LR890422
Organism Klebsiella pneumoniae isolate INF350-sc-2280174

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag JMW70_RS22695 Protein ID WP_004178461.1
Coordinates 4676670..4677203 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JMW70_RS22700 Protein ID WP_002887278.1
Coordinates 4677214..4677480 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW70_RS22690 4675501..4676622 + 1122 WP_002887282.1 cupin domain-containing protein -
JMW70_RS22695 4676670..4677203 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
JMW70_RS22700 4677214..4677480 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JMW70_RS22705 4677583..4679016 - 1434 WP_009484674.1 Cu(+)/Ag(+) sensor histidine kinase -
JMW70_RS22710 4679006..4679689 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
JMW70_RS22715 4679861..4681246 + 1386 WP_009484676.1 efflux transporter outer membrane subunit -
JMW70_RS22720 4681264..4681608 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T290964 WP_004178461.1 NZ_LR890422:c4677203-4676670 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT290964 WP_002887278.1 NZ_LR890422:c4677480-4677214 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References