Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 45039..45682 Replicon plasmid 2
Accession NZ_LR890411
Organism Escherichia coli isolate MSB2_1A-sc-2280429

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag JMW04_RS23945 Protein ID WP_001034044.1
Coordinates 45266..45682 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag JMW04_RS23940 Protein ID WP_001261286.1
Coordinates 45039..45269 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW04_RS23925 40176..40406 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JMW04_RS23930 40403..40819 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
JMW04_RS23935 40864..44658 - 3795 WP_001144732.1 hypothetical protein -
JMW04_RS23940 45039..45269 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JMW04_RS23945 45266..45682 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
JMW04_RS23950 45757..47322 + 1566 WP_001128474.1 AAA family ATPase -
JMW04_RS23955 47307..48329 + 1023 WP_000361402.1 helicase UvrD -
JMW04_RS23960 48583..49280 - 698 Protein_55 IS1 family transposase -
JMW04_RS23965 49583..50280 + 698 Protein_56 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IId / blaTEM-1B - 1..52208 52208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T290936 WP_001034044.1 NZ_LR890411:45266-45682 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT290936 WP_001261286.1 NZ_LR890411:45039-45269 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References