Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 35687..36212 Replicon plasmid 2
Accession NZ_LR890411
Organism Escherichia coli isolate MSB2_1A-sc-2280429

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JMW04_RS23895 Protein ID WP_001159868.1
Coordinates 35687..35992 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JMW04_RS23900 Protein ID WP_000813634.1
Coordinates 35994..36212 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW04_RS23880 31597..32763 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
JMW04_RS23885 33351..34106 - 756 WP_000852146.1 replication initiation protein RepE -
JMW04_RS23890 34880..35686 - 807 WP_000016982.1 site-specific integrase -
JMW04_RS23895 35687..35992 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JMW04_RS23900 35994..36212 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JMW04_RS23905 36779..37291 + 513 WP_000151784.1 hypothetical protein -
JMW04_RS23910 37325..38458 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
JMW04_RS23915 38625..39398 - 774 WP_000905949.1 hypothetical protein -
JMW04_RS23920 39411..39911 - 501 WP_001773886.1 hypothetical protein -
JMW04_RS23925 40176..40406 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JMW04_RS23930 40403..40819 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IId / blaTEM-1B - 1..52208 52208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T290934 WP_001159868.1 NZ_LR890411:c35992-35687 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT290934 WP_000813634.1 NZ_LR890411:c36212-35994 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References