Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1529113..1529696 Replicon chromosome
Accession NZ_LR890410
Organism Escherichia coli isolate MSB2_1A-sc-2280429

Toxin (Protein)


Gene name mazF Uniprot ID S1PVD8
Locus tag JMW04_RS07330 Protein ID WP_000254749.1
Coordinates 1529113..1529448 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JMW04_RS07335 Protein ID WP_000581937.1
Coordinates 1529448..1529696 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW04_RS07315 1524999..1526297 - 1299 WP_000036723.1 phosphopyruvate hydratase -
JMW04_RS07320 1526385..1528022 - 1638 WP_001774030.1 CTP synthase (glutamine hydrolyzing) -
JMW04_RS07325 1528250..1529041 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JMW04_RS07330 1529113..1529448 - 336 WP_000254749.1 endoribonuclease MazF Toxin
JMW04_RS07335 1529448..1529696 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JMW04_RS07340 1529774..1532008 - 2235 WP_000226797.1 GTP diphosphokinase -
JMW04_RS07345 1532056..1533357 - 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12137.06 Da        Isoelectric Point: 8.7218

>T290915 WP_000254749.1 NZ_LR890410:c1529448-1529113 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT290915 WP_000581937.1 NZ_LR890410:c1529696-1529448 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PVD8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References