Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 2472776..2473299 Replicon chromosome
Accession NC_020533
Organism Staphylococcus aureus subsp. aureus ST228

Toxin (Protein)


Gene name relE Uniprot ID A0A3A5LQI3
Locus tag SAI4T8_RS12525 Protein ID WP_000074049.1
Coordinates 2472776..2473042 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID W8TQT5
Locus tag SAI4T8_RS12530 Protein ID WP_000587616.1
Coordinates 2473042..2473299 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAI4T8_RS12505 2468083..2469270 - 1188 WP_000026189.1 MFS transporter -
SAI4T8_RS12510 2469666..2470442 - 777 WP_041174228.1 iron export ABC transporter permease subunit FetB -
SAI4T8_RS12515 2470435..2471097 - 663 WP_000923526.1 ATP-binding cassette domain-containing protein -
SAI4T8_RS12520 2471345..2472421 - 1077 WP_031870304.1 M42 family metallopeptidase -
SAI4T8_RS12525 2472776..2473042 - 267 WP_000074049.1 Txe/YoeB family addiction module toxin Toxin
SAI4T8_RS12530 2473042..2473299 - 258 WP_000587616.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
SAI4T8_RS12540 2473658..2474113 + 456 WP_000732076.1 DUF1307 domain-containing protein -
SAI4T8_RS12545 2474281..2475858 - 1578 WP_000143494.1 FMN-binding glutamate synthase family protein -
SAI4T8_RS12550 2476054..2476803 + 750 WP_000072461.1 CPBP family intramembrane metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(4-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-68)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10676.24 Da        Isoelectric Point: 10.1597

>T29090 WP_000074049.1 NC_020533:c2473042-2472776 [Staphylococcus aureus subsp. aureus ST228]
MSNYTVKIKNSAKSDLRKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Download         Length: 267 bp

>T29090 NC_020533:c2473042-2472776 [Staphylococcus aureus subsp. aureus ST228]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9774.73 Da        Isoelectric Point: 4.1353

>AT29090 WP_000587616.1 NC_020533:c2473299-2473042 [Staphylococcus aureus subsp. aureus ST228]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

Download         Length: 258 bp

>AT29090 NC_020533:c2473299-2473042 [Staphylococcus aureus subsp. aureus ST228]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A5LQI3

Antitoxin

Source ID Structure
PDB 7V6W
PDB 7V5Z
PDB 7V5Y
AlphaFold DB W8TQT5

References