Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3067782..3068566 Replicon chromosome
Accession NZ_LR890405
Organism Escherichia coli isolate MSB1_7C-sc-2280317

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag JMW09_RS14845 Protein ID WP_000613626.1
Coordinates 3068072..3068566 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag JMW09_RS14840 Protein ID WP_001110447.1
Coordinates 3067782..3068075 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW09_RS14830 3062932..3063891 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
JMW09_RS14835 3064464..3067649 + 3186 WP_000827427.1 ribonuclease E -
JMW09_RS14840 3067782..3068075 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
JMW09_RS14845 3068072..3068566 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
JMW09_RS14850 3068661..3069614 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
JMW09_RS14855 3069626..3071269 - 1644 WP_000096524.1 flagellar hook-associated protein FlgK -
JMW09_RS14860 3071335..3072276 - 942 WP_001309406.1 flagellar assembly peptidoglycan hydrolase FlgJ -
JMW09_RS14865 3072276..3073373 - 1098 WP_000589320.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T290895 WP_000613626.1 NZ_LR890405:3068072-3068566 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT290895 WP_001110447.1 NZ_LR890405:3067782-3068075 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References