Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 164315..164958 Replicon plasmid 2
Accession NZ_LR890396
Organism Klebsiella pneumoniae isolate INF044-sc-2279940

Toxin (Protein)


Gene name vagD Uniprot ID A0A5Q9LNG2
Locus tag JMW67_RS26885 Protein ID WP_048983057.1
Coordinates 164315..164731 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag JMW67_RS26890 Protein ID WP_001261276.1
Coordinates 164728..164958 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW67_RS26870 159363..161471 - 2109 WP_016338368.1 peptidase domain-containing ABC transporter -
JMW67_RS26875 161461..162738 - 1278 WP_016338369.1 HlyD family secretion protein -
JMW67_RS26880 163397..164274 + 878 Protein_169 restriction endonuclease -
JMW67_RS26885 164315..164731 - 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin Toxin
JMW67_RS26890 164728..164958 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JMW67_RS26895 165873..166181 + 309 WP_004152336.1 hypothetical protein -
JMW67_RS26900 166209..166538 + 330 WP_004152337.1 hypothetical protein -
JMW67_RS26905 166564..166962 + 399 WP_004171440.1 hypothetical protein -
JMW67_RS26910 166969..167301 + 333 WP_004152339.1 hypothetical protein -
JMW67_RS26915 167354..168058 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
JMW67_RS26920 168070..168726 - 657 WP_000113282.1 tetracycline resistance transcriptional repressor TetR(D) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qacE / sul1 / mph(A) / aph(3')-Ia / tet(D) / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / catA1 - 1..227214 227214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15022.52 Da        Isoelectric Point: 9.2957

>T290880 WP_048983057.1 NZ_LR890396:c164731-164315 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Download         Length: 417 bp

>T290880 NZ_LR890396:c164731-164315 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT290880 WP_001261276.1 NZ_LR890396:c164958-164728 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT290880 NZ_LR890396:c164958-164728 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNG2

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References