Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 154293..155029 Replicon plasmid 2
Accession NZ_LR890396
Organism Klebsiella pneumoniae isolate INF044-sc-2279940

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JMW67_RS26815 Protein ID WP_003026803.1
Coordinates 154293..154775 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JMW67_RS26820 Protein ID WP_003026799.1
Coordinates 154763..155029 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW67_RS26800 149930..150892 + 963 WP_004152113.1 M48 family metalloprotease -
JMW67_RS26805 150980..152234 + 1255 Protein_154 IS3 family transposase -
JMW67_RS26810 152735..154081 - 1347 WP_020314316.1 ISNCY family transposase -
JMW67_RS26815 154293..154775 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JMW67_RS26820 154763..155029 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JMW67_RS26825 155236..155430 + 195 WP_019725033.1 hypothetical protein -
JMW67_RS26830 155474..155704 - 231 WP_019725034.1 hypothetical protein -
JMW67_RS26835 155718..155921 - 204 WP_019725040.1 hemolysin expression modulator Hha -
JMW67_RS26840 155955..156323 - 369 WP_015065502.1 hypothetical protein -
JMW67_RS26845 156367..156861 - 495 WP_009310053.1 hypothetical protein -
JMW67_RS26850 156892..157464 - 573 WP_015065504.1 hypothetical protein -
JMW67_RS26855 157461..157709 - 249 WP_019725036.1 hypothetical protein -
JMW67_RS26860 158276..158620 + 345 WP_058650054.1 hypothetical protein -
JMW67_RS26865 158728..159363 - 636 WP_016338367.1 CPBP family intramembrane metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qacE / sul1 / mph(A) / aph(3')-Ia / tet(D) / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / catA1 - 1..227214 227214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T290879 WP_003026803.1 NZ_LR890396:c154775-154293 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT290879 WP_003026799.1 NZ_LR890396:c155029-154763 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References