Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2092838..2093352 Replicon chromosome
Accession NC_020533
Organism Staphylococcus aureus subsp. aureus ST228

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag SAI4T8_RS10500 Protein ID WP_000581792.1
Coordinates 2092838..2093047 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag SAI4T8_RS10505 Protein ID WP_000138932.1
Coordinates 2093059..2093352 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAI4T8_RS10480 2088346..2089704 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
SAI4T8_RS10485 2089719..2090789 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
SAI4T8_RS10490 2091107..2092309 + 1203 WP_001109939.1 rod shape-determining protein RodA -
SAI4T8_RS10495 2092573..2092710 - 138 WP_000828354.1 hypothetical protein -
SAI4T8_RS10500 2092838..2093047 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
SAI4T8_RS10505 2093059..2093352 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
SAI4T8_RS10510 2093518..2095002 + 1485 WP_000571549.1 cardiolipin synthase -
SAI4T8_RS10515 2095030..2095677 + 648 WP_001187630.1 HD domain-containing protein -
SAI4T8_RS10525 2096490..2097362 - 873 WP_000725802.1 membrane protein insertase YidC -
SAI4T8_RS10530 2097448..2098089 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T29084 WP_000581792.1 NC_020533:c2093047-2092838 [Staphylococcus aureus subsp. aureus ST228]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T29084 NC_020533:c2093047-2092838 [Staphylococcus aureus subsp. aureus ST228]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT29084 WP_000138932.1 NC_020533:c2093352-2093059 [Staphylococcus aureus subsp. aureus ST228]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT29084 NC_020533:c2093352-2093059 [Staphylococcus aureus subsp. aureus ST228]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References