290712

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 117207..117732 Replicon plasmid 2
Accession NZ_LR890335
Organism Escherichia coli isolate MINF_9A-sc-2280436

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JMW07_RS25445 Protein ID WP_001159868.1
Coordinates 117427..117732 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JMW07_RS25440 Protein ID WP_000813634.1
Coordinates 117207..117425 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW07_RS25410 112600..113016 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
JMW07_RS25415 113013..113243 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JMW07_RS25420 113508..114008 + 501 WP_000528931.1 hypothetical protein -
JMW07_RS25425 114021..114794 + 774 WP_000905949.1 hypothetical protein -
JMW07_RS25430 114961..116094 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
JMW07_RS25435 116128..116640 - 513 WP_000151784.1 hypothetical protein -
JMW07_RS25440 117207..117425 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JMW07_RS25445 117427..117732 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JMW07_RS25450 117733..118539 + 807 WP_000016982.1 site-specific integrase -
JMW07_RS25455 119313..120068 + 756 WP_024946706.1 replication initiation protein RepE -
JMW07_RS25460 120502..121199 - 698 Protein_153 IS1 family transposase -
JMW07_RS25465 121555..122469 + 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 / sitABCD iucA / iucB / iucC / iucD / iutA 1..139312 139312
- flank IS/Tn sitABCD - 120502..125004 4502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T290712 WP_001159868.1 NZ_LR890335:117427-117732 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT290712 WP_000813634.1 NZ_LR890335:117207-117425 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References