Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2092822..2093336 Replicon chromosome
Accession NC_020532
Organism Staphylococcus aureus subsp. aureus ST228

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag SAI3T3_RS10485 Protein ID WP_000581792.1
Coordinates 2092822..2093031 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag SAI3T3_RS10490 Protein ID WP_000138932.1
Coordinates 2093043..2093336 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAI3T3_RS10465 2088330..2089688 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
SAI3T3_RS10470 2089703..2090773 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
SAI3T3_RS10475 2091091..2092293 + 1203 WP_001109939.1 rod shape-determining protein RodA -
SAI3T3_RS14940 2092557..2092694 - 138 WP_000828354.1 hypothetical protein -
SAI3T3_RS10485 2092822..2093031 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
SAI3T3_RS10490 2093043..2093336 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
SAI3T3_RS10495 2093502..2094986 + 1485 WP_000571549.1 cardiolipin synthase -
SAI3T3_RS10500 2095014..2095661 + 648 WP_001187630.1 HD domain-containing protein -
SAI3T3_RS10510 2096474..2097346 - 873 WP_000725802.1 membrane protein insertase YidC -
SAI3T3_RS10515 2097432..2098073 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T29069 WP_000581792.1 NC_020532:c2093031-2092822 [Staphylococcus aureus subsp. aureus ST228]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T29069 NC_020532:c2093031-2092822 [Staphylococcus aureus subsp. aureus ST228]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT29069 WP_000138932.1 NC_020532:c2093336-2093043 [Staphylococcus aureus subsp. aureus ST228]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT29069 NC_020532:c2093336-2093043 [Staphylococcus aureus subsp. aureus ST228]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References