Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 52351..52876 Replicon plasmid 2
Accession NZ_LR890327
Organism Escherichia coli isolate MINF_1A-sc-2280431

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JMV91_RS25515 Protein ID WP_001159868.1
Coordinates 52571..52876 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JMV91_RS25510 Protein ID WP_000813634.1
Coordinates 52351..52569 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV91_RS25485 47460..48329 - 870 WP_001586225.1 hypothetical protein -
JMV91_RS25490 48331..49233 - 903 WP_024187432.1 hypothetical protein -
JMV91_RS25495 49234..49419 - 186 WP_001586223.1 hypothetical protein -
JMV91_RS25500 49712..50644 - 933 WP_000991831.1 hypothetical protein -
JMV91_RS25505 50648..51643 - 996 WP_000246635.1 hypothetical protein -
JMV91_RS25510 52351..52569 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JMV91_RS25515 52571..52876 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JMV91_RS25520 52877..53683 + 807 WP_000016982.1 site-specific integrase -
JMV91_RS25525 54457..55212 + 756 WP_000852146.1 replication initiation protein RepE -
JMV91_RS25530 55800..56966 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / aadA5 / qacE / sul1 / mph(A) / aac(3)-IId / sul2 / aph(3'')-Ib / aph(6)-Id - 1..98336 98336


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T290671 WP_001159868.1 NZ_LR890327:52571-52876 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT290671 WP_000813634.1 NZ_LR890327:52351-52569 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References