Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 37588..38391 Replicon plasmid 2
Accession NZ_LR890327
Organism Escherichia coli isolate MINF_1A-sc-2280431

Toxin (Protein)


Gene name KacT Uniprot ID G3CAI8
Locus tag JMV91_RS25435 Protein ID WP_000348883.1
Coordinates 37861..38391 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID G3CAI7
Locus tag JMV91_RS25430 Protein ID WP_001275013.1
Coordinates 37588..37857 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV91_RS25405 33733..34001 + 269 Protein_43 type II toxin-antitoxin system ParD family antitoxin -
JMV91_RS25410 34117..34278 + 162 WP_001440647.1 type II toxin-antitoxin system RelE/ParE family toxin -
JMV91_RS25415 34345..34527 + 183 WP_042004909.1 hypothetical protein -
JMV91_RS25420 34648..35388 + 741 WP_001586233.1 tyrosine-type recombinase/integrase -
JMV91_RS25425 35711..36700 - 990 WP_123002471.1 RepB family plasmid replication initiator protein -
JMV91_RS25430 37588..37857 + 270 WP_001275013.1 DUF1778 domain-containing protein Antitoxin
JMV91_RS25435 37861..38391 + 531 WP_000348883.1 GNAT family N-acetyltransferase Toxin
JMV91_RS25440 38392..38609 + 218 Protein_50 transposase -
JMV91_RS25445 38614..38889 - 276 WP_001403920.1 type II toxin-antitoxin system VapC family toxin -
JMV91_RS25450 38978..39208 - 231 WP_001261274.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JMV91_RS25455 39407..42028 - 2622 WP_001586230.1 hypothetical protein -
JMV91_RS25460 42419..42904 + 486 WP_001403958.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / aadA5 / qacE / sul1 / mph(A) / aac(3)-IId / sul2 / aph(3'')-Ib / aph(6)-Id - 1..98336 98336


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20321.20 Da        Isoelectric Point: 6.4889

>T290669 WP_000348883.1 NZ_LR890327:37861-38391 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10229.63 Da        Isoelectric Point: 6.4702

>AT290669 WP_001275013.1 NZ_LR890327:37588-37857 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAI8

Antitoxin

Source ID Structure
AlphaFold DB G3CAI7

References