Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1124224..1125061 Replicon chromosome
Accession NZ_LR890326
Organism Escherichia coli isolate MINF_1A-sc-2280431

Toxin (Protein)


Gene name itaT Uniprot ID V0SED1
Locus tag JMV91_RS05315 Protein ID WP_000227787.1
Coordinates 1124224..1124766 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag JMV91_RS05320 Protein ID WP_001353405.1
Coordinates 1124750..1125061 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV91_RS05295 1119764..1120675 - 912 WP_000705871.1 2-dehydropantoate 2-reductase -
JMV91_RS05300 1120843..1121334 + 492 WP_001138904.1 nucleotide binding protein YajQ -
JMV91_RS05305 1121462..1122826 - 1365 WP_001000978.1 MFS transporter -
JMV91_RS05310 1123233..1124168 + 936 WP_012311701.1 sel1 repeat family protein -
JMV91_RS05315 1124224..1124766 - 543 WP_000227787.1 GNAT family N-acetyltransferase Toxin
JMV91_RS05320 1124750..1125061 - 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
JMV91_RS05325 1125246..1126136 - 891 WP_000971336.1 heme o synthase -
JMV91_RS05330 1126148..1126477 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JMV91_RS05335 1126477..1127091 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JMV91_RS05340 1127081..1129072 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JMV91_RS05345 1129094..1130041 - 948 WP_001239436.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19946.24 Da        Isoelectric Point: 8.8951

>T290653 WP_000227787.1 NZ_LR890326:c1124766-1124224 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT290653 WP_001353405.1 NZ_LR890326:c1125061-1124750 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GE43

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References