Detailed information of TA system TA290633

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 657585..658264
• Replicon: chromosome
• Accession: NZ_LR890323
• Organism: Klebsiella pneumoniae isolate INF348-sc-2280170

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: JMW45_RS03300
• Protein ID: WP_201522333.1
• Coordinates: 657923..658264 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: JMW45_RS03295
• Protein ID: WP_201522332.1
• Coordinates: 657585..657902 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW45_RS03250	652933..653550	+	618	Protein_638	DUF945 domain-containing protein	-
JMW45_RS03255	653759..654469	+	711	WP_201522328.1	DeoR family transcriptional regulator	-
JMW45_RS03260	654495..655031	+	537	WP_201522329.1	DUF4339 domain-containing protein	-
JMW45_RS03265	655073..655510	+	438	WP_023301392.1	hypothetical protein	-
JMW45_RS03270	655577..655987	+	411	WP_064411433.1	hypothetical protein	-
JMW45_RS03275	656065..656301	+	237	WP_064411434.1	DUF905 domain-containing protein	-
JMW45_RS03280	656387..656845	+	459	WP_023301394.1	antirestriction protein	-
JMW45_RS03285	656854..657336	+	483	WP_201522330.1	RadC family protein	-
JMW45_RS03290	657345..657566	+	222	WP_201522331.1	DUF987 domain-containing protein	-
JMW45_RS03295	657585..657902	+	318	WP_201522332.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JMW45_RS03300	657923..658264	+	342	WP_201522333.1	TA system toxin CbtA family protein	Toxin
JMW45_RS03310	658626..659132	+	507	WP_002917636.1	G/U mismatch-specific DNA glycosylase	-
JMW45_RS03315	659231..661072	-	1842	WP_004174339.1	RNA polymerase sigma factor RpoD	-
JMW45_RS03320	661291..663036	-	1746	WP_201522334.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	635891..658264	22373

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12910.01 Da
• Isoelectric Point: 10.3872

>T290633 WP_201522333.1 NZ_LR890323:657923-658264 [Klebsiella pneumoniae]
MKTLSATISRAAKPCLSPVAVWQMLLTRLLEKHYALTLNDTPFGDERVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11889.36 Da
• Isoelectric Point: 6.6246

>AT290633 WP_201522332.1 NZ_LR890323:657585-657902 [Klebsiella pneumoniae]
MSNITWGLQRDTTPRLGARLVQEGNQLHYLADRASITGKFSDAECRKLDETFPHFISQMESMLTTGELNPRHAHCVTLYH
NGFTCEADTFGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp