Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1219876..1220660 Replicon chromosome
Accession NZ_LR890299
Organism Escherichia coli isolate MSB1_3B-sc-2280406

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag JM188_RS06050 Protein ID WP_000613626.1
Coordinates 1220166..1220660 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag JM188_RS06045 Protein ID WP_001110447.1
Coordinates 1219876..1220169 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JM188_RS06035 1215026..1215985 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
JM188_RS06040 1216558..1219743 + 3186 WP_001550951.1 ribonuclease E -
JM188_RS06045 1219876..1220169 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
JM188_RS06050 1220166..1220660 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
JM188_RS06055 1220755..1221708 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
JM188_RS06060 1221720..1223363 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
JM188_RS06065 1223429..1224370 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
JM188_RS06070 1224370..1225467 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T290553 WP_000613626.1 NZ_LR890299:1220166-1220660 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT290553 WP_001110447.1 NZ_LR890299:1219876-1220169 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References