Detailed information of TA system TA290544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 66167..66689
• Replicon: plasmid 2
• Accession: NZ_LR890292
• Organism: Klebsiella pneumoniae strain KSB1_1B

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: JMW65_RS25935
• Protein ID: WP_004187019.1
• Coordinates: 66167..66451 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: JMW65_RS25940
• Protein ID: WP_004187025.1
• Coordinates: 66441..66689 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW65_RS25910	61386..61850	-	465	WP_004187010.1	hypothetical protein	-
JMW65_RS25915	63393..63917	+	525	Protein_59	hypothetical protein	-
JMW65_RS25920	63934..64417	+	484	Protein_60	hypothetical protein	-
JMW65_RS25925	64415..64714	+	300	Protein_61	conjugal transfer protein TraH	-
JMW65_RS25930	64796..66151	+	1356	WP_004187017.1	ISNCY family transposase	-
JMW65_RS25935	66167..66451	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JMW65_RS25940	66441..66689	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
JMW65_RS25945	66979..67403	-	425	Protein_65	IS1 family transposase	-
JMW65_RS25950	67591..67707	-	117	Protein_66	transposase domain-containing protein	-
JMW65_RS25955	67652..67915	-	264	WP_004187027.1	transposase	-
JMW65_RS25960	67930..68193	+	264	WP_004118208.1	hypothetical protein	-
JMW65_RS25965	68437..68718	+	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
JMW65_RS25970	68753..69322	+	570	WP_004152117.1	small heat shock protein sHSP20	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..133165	133165
	-	flank	IS/Tn	-	-	67128..67352	224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T290544 WP_004187019.1 NZ_LR890292:c66451-66167 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT290544 WP_004187025.1 NZ_LR890292:c66689-66441 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4