Detailed information of TA system TA029053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2071263..2071792
• Replicon: chromosome
• Accession: NC_020529
• Organism: Staphylococcus aureus subsp. aureus ST228

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAI1T1_RS10390
• Protein ID: WP_000621175.1
• Coordinates: 2071263..2071625 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAI1T1_RS10395
• Protein ID: WP_000948331.1
• Coordinates: 2071622..2071792 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAI1T1_RS10365	2068241..2069011	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
SAI1T1_RS10370	2068986..2069465	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAI1T1_RS10375	2069467..2069793	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAI1T1_RS10380	2069912..2070913	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAI1T1_RS10390	2071263..2071625	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAI1T1_RS10395	2071622..2071792	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAI1T1_RS10400	2071877..2073025	-	1149	WP_001281154.1	alanine racemase	-
SAI1T1_RS10405	2073091..2073450	-	360	WP_000581197.1	holo-ACP synthase	-
SAI1T1_RS10410	2073454..2073945	-	492	WP_001205912.1	PH domain-containing protein	-
SAI1T1_RS10415	2073932..2075515	-	1584	WP_001294626.1	PH domain-containing protein	-
SAI1T1_RS10420	2075508..2075987	-	480	WP_001287087.1	hypothetical protein	-
SAI1T1_RS10425	2076195..2076755	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T29053 WP_000621175.1 NC_020529:c2071625-2071263 [Staphylococcus aureus subsp. aureus ST228]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T29053 NC_020529:c2071625-2071263 [Staphylococcus aureus subsp. aureus ST228]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT29053 WP_000948331.1 NC_020529:c2071792-2071622 [Staphylococcus aureus subsp. aureus ST228]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT29053 NC_020529:c2071792-2071622 [Staphylococcus aureus subsp. aureus ST228]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1