Detailed information of TA system TA290499

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1842426..1843261
• Replicon: chromosome
• Accession: NZ_LR890288
• Organism: Escherichia coli isolate MSB1_1A-sc-2280383

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JMW08_RS08750
• Protein ID: WP_042046912.1
• Coordinates: 1842426..1842803 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JMW08_RS08755
• Protein ID: WP_135145692.1
• Coordinates: 1842893..1843261 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW08_RS08715	1837946..1838419	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
JMW08_RS08720	1838617..1839675	+	1059	WP_001200895.1	FUSC family protein	-
JMW08_RS08725	1839847..1840176	+	330	WP_000450409.1	DUF496 family protein	-
JMW08_RS08730	1840277..1840459	-	183	WP_024198358.1	ethanolamine utilization protein	-
JMW08_RS08735	1840913..1841302	-	390	WP_077875581.1	transposase	-
JMW08_RS08740	1842100..1842213	-	114	WP_032140621.1	DUF957 domain-containing protein	-
JMW08_RS08745	1842226..1842429	-	204	WP_000761705.1	hypothetical protein	-
JMW08_RS08750	1842426..1842803	-	378	WP_042046912.1	TA system toxin CbtA family protein	Toxin
JMW08_RS08755	1842893..1843261	-	369	WP_135145692.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JMW08_RS08760	1843424..1843645	-	222	WP_000692345.1	DUF987 domain-containing protein	-
JMW08_RS08765	1843708..1844184	-	477	WP_001186774.1	RadC family protein	-
JMW08_RS08770	1844200..1844673	-	474	WP_000855059.1	antirestriction protein	-
JMW08_RS08775	1844797..1845543	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
JMW08_RS08780	1845558..1847099	-	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14020.96 Da
• Isoelectric Point: 7.8276

>T290499 WP_042046912.1 NZ_LR890288:c1842803-1842426 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13545.30 Da
• Isoelectric Point: 6.4764

>AT290499 WP_135145692.1 NZ_LR890288:c1843261-1842893 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPHHQHTVTLYAKGLTCEADTLGSCGYAYLAVYPTPETKK

Nucleotide

• Length: 369 bp