Detailed information of TA system TA029049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1886437..1887213
• Replicon: chromosome
• Accession: NC_020529
• Organism: Staphylococcus aureus subsp. aureus ST228

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: SAI1T1_RS14795
• Protein ID: WP_000031108.1
• Coordinates: 1886437..1886589 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: SAI1T1_RS09215
• Protein ID: WP_001251224.1
• Coordinates: 1886614..1887213 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAI1T1_RS09195	1882639..1883460	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
SAI1T1_RS09200	1883913..1885298	-	1386	WP_000116228.1	class II fumarate hydratase	-
SAI1T1_RS09205	1885494..1885889	-	396	WP_000901023.1	hypothetical protein	-
SAI1T1_RS14795	1886437..1886589	-	153	WP_000031108.1	hypothetical protein	Toxin
SAI1T1_RS09215	1886614..1887213	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
SAI1T1_RS09220	1887372..1887842	-	471	WP_015445875.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
SAI1T1_RS09225	1887847..1888974	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
SAI1T1_RS09230	1889125..1889847	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
SAI1T1_RS09235	1889840..1891297	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T29049 WP_000031108.1 NC_020529:c1886589-1886437 [Staphylococcus aureus subsp. aureus ST228]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T29049 NC_020529:c1886589-1886437 [Staphylococcus aureus subsp. aureus ST228]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT29049 WP_001251224.1 NC_020529:c1887213-1886614 [Staphylococcus aureus subsp. aureus ST228]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT29049 NC_020529:c1887213-1886614 [Staphylococcus aureus subsp. aureus ST228]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4