Detailed information of TA system TA029044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2889835..2890466
• Replicon: chromosome
• Accession: NC_020528
• Organism: Sinorhizobium meliloti 2011

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SM2011_RS28060
• Protein ID: WP_010970128.1
• Coordinates: 2889835..2890233 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92MD9
• Locus tag: SM2011_RS28065
• Protein ID: WP_010970129.1
• Coordinates: 2890233..2890466 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM2011_RS28025	2884915..2885217	-	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SM2011_RS28030	2885214..2885489	-	276	WP_010970123.1	type II toxin-antitoxin system ParD family antitoxin	-
SM2011_RS36305	2885777..2885926	+	150	WP_010970124.1	hypothetical protein	-
SM2011_RS28040	2885923..2886858	+	936	WP_010970125.1	site-specific tyrosine recombinase XerD	-
SM2011_RS28045	2886922..2887875	+	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
SM2011_RS28050	2888120..2889562	+	1443	WP_010970126.1	murein L,D-transpeptidase	-
SM2011_RS28055	2889562..2889801	+	240	WP_010970127.1	sulfurtransferase TusA family protein	-
SM2011_RS28060	2889835..2890233	-	399	WP_010970128.1	type II toxin-antitoxin system toxin VapC	Toxin
SM2011_RS28065	2890233..2890466	-	234	WP_010970129.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SM2011_RS28070	2890575..2891720	-	1146	WP_010970130.1	GTP-binding protein	-
SM2011_RS28075	2891734..2892816	-	1083	WP_010970131.1	D-alanyl-D-alanine carboxypeptidase	-
SM2011_RS28080	2893068..2894231	+	1164	WP_010970132.1	amidohydrolase	-
SM2011_RS28085	2894254..2894718	+	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14859.16 Da
• Isoelectric Point: 6.6329

>T29044 WP_010970128.1 NC_020528:c2890233-2889835 [Sinorhizobium meliloti 2011]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSAWRVENLTAIEHFVARLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T29044 NC_020528:c2890233-2889835 [Sinorhizobium meliloti 2011]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAAAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCCGAAAAATCGGCCTGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACGGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCTCATGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACCAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8673.87 Da
• Isoelectric Point: 8.9167

>AT29044 WP_010970129.1 NC_020528:c2890466-2890233 [Sinorhizobium meliloti 2011]
MASSTVFISNRSQAVRLPKAVAFPEGVHQVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT29044 NC_020528:c2890466-2890233 [Sinorhizobium meliloti 2011]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAGGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATCAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q92MD9