Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3587507..3588083 Replicon chromosome
Accession NZ_LR890270
Organism Escherichia coli isolate MINF_7C-sc-2280452

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag JMW01_RS16905 Protein ID WP_001297643.1
Coordinates 3587507..3587794 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag JMW01_RS16910 Protein ID WP_000063156.1
Coordinates 3587781..3588083 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW01_RS16890 3583744..3585024 - 1281 WP_001037390.1 DUF445 domain-containing protein -
JMW01_RS16895 3585065..3586297 - 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
JMW01_RS16900 3586612..3587308 + 697 Protein_3294 IS1 family transposase -
JMW01_RS16905 3587507..3587794 + 288 WP_001297643.1 BrnT family toxin Toxin
JMW01_RS16910 3587781..3588083 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
JMW01_RS16915 3588117..3589073 - 957 WP_001297640.1 GTPase -
JMW01_RS16920 3589084..3589287 - 204 WP_000467859.1 YbdD/YjiX family protein -
JMW01_RS16925 3589337..3591487 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3587099..3587308 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T290437 WP_001297643.1 NZ_LR890270:3587507-3587794 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT290437 WP_000063156.1 NZ_LR890270:3587781-3588083 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References