Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3269212..3269709 Replicon chromosome
Accession NZ_LR890270
Organism Escherichia coli isolate MINF_7C-sc-2280452

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JMW01_RS15310 Protein ID WP_001173343.1
Coordinates 3269536..3269709 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JMW01_RS15305 Protein ID WP_000398619.1
Coordinates 3269212..3269508 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW01_RS15285 3265941..3266660 - 720 WP_000611288.1 two-component system response regulator DcuR -
JMW01_RS15290 3266657..3268288 - 1632 WP_001216477.1 sensor histidine kinase -
JMW01_RS15295 3268469..3268699 + 231 WP_000373291.1 (4Fe-4S)-binding protein -
JMW01_RS15300 3268711..3268983 + 273 WP_000405647.1 N-acetyltransferase -
JMW01_RS15305 3269212..3269508 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JMW01_RS15310 3269536..3269709 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JMW01_RS15315 3269827..3271344 - 1518 WP_000003806.1 lysine--tRNA ligase -
JMW01_RS15320 3271581..3273038 - 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T290435 WP_001173343.1 NZ_LR890270:3269536-3269709 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT290435 WP_000398619.1 NZ_LR890270:3269212-3269508 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References