29043

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacT-ataR/DUF1778(antitoxin)
Location 2842286..2843052 Replicon chromosome
Accession NC_020528
Organism Sinorhizobium meliloti 2011

Toxin (Protein)


Gene name tacT Uniprot ID H0G6D3
Locus tag SM2011_RS27795 Protein ID WP_003533488.1
Coordinates 2842570..2843052 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID Q92MI4
Locus tag SM2011_RS27790 Protein ID WP_010970089.1
Coordinates 2842286..2842570 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SM2011_RS27775 2837756..2839144 + 1389 WP_003533497.1 lipopolysaccharide biosynthesis protein -
SM2011_RS27780 2839148..2840419 + 1272 WP_010970087.1 GNAT family N-acetyltransferase -
SM2011_RS27785 2840435..2842081 - 1647 WP_010970088.1 AMP-binding protein -
SM2011_RS27790 2842286..2842570 + 285 WP_010970089.1 DUF1778 domain-containing protein Antitoxin
SM2011_RS27795 2842570..2843052 + 483 WP_003533488.1 GNAT family N-acetyltransferase Toxin
SM2011_RS27805 2843517..2844026 + 510 WP_010970090.1 disulfide bond formation protein B -
SM2011_RS27810 2844058..2844615 - 558 WP_003533486.1 HNH endonuclease -
SM2011_RS27815 2844721..2845368 - 648 WP_010970091.1 DNA-3-methyladenine glycosylase -
SM2011_RS27820 2845521..2846417 + 897 WP_010970092.1 tRNA glutamyl-Q(34) synthetase GluQRS -
SM2011_RS27825 2846436..2847311 - 876 WP_010970093.1 YihY/virulence factor BrkB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-142)

Antitoxin

Vibrio phage ICP1, Orf50

(15-79)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17463.88 Da        Isoelectric Point: 7.3827

>T29043 WP_003533488.1 NC_020528:2842570-2843052 [Sinorhizobium meliloti 2011]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Download         Length: 483 bp

>T29043 NC_020528:2842570-2843052 [Sinorhizobium meliloti 2011]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10741.10 Da        Isoelectric Point: 7.3175

>AT29043 WP_010970089.1 NC_020528:2842286-2842570 [Sinorhizobium meliloti 2011]
MATKSSADTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Download         Length: 285 bp

>AT29043 NC_020528:2842286-2842570 [Sinorhizobium meliloti 2011]
ATGGCCACGAAATCGTCGGCTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H0G6D3

Antitoxin

Source ID Structure
AlphaFold DB Q92MI4

References