Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4633597..4634407 Replicon chromosome
Accession NZ_LR890245
Organism Klebsiella pneumoniae isolate INF174-sc-2280034

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag JMV74_RS22450 Protein ID WP_004178461.1
Coordinates 4633597..4634130 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JMV74_RS22455 Protein ID WP_002887278.1
Coordinates 4634141..4634407 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV74_RS22445 4632428..4633549 + 1122 WP_002887282.1 cupin domain-containing protein -
JMV74_RS22450 4633597..4634130 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
JMV74_RS22455 4634141..4634407 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JMV74_RS22460 4634510..4635943 - 1434 WP_009484674.1 Cu(+)/Ag(+) sensor histidine kinase -
JMV74_RS22465 4635933..4636616 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
JMV74_RS22470 4636788..4638173 + 1386 WP_009484676.1 efflux transporter outer membrane subunit -
JMV74_RS22475 4638191..4638535 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T290395 WP_004178461.1 NZ_LR890245:c4634130-4633597 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT290395 WP_002887278.1 NZ_LR890245:c4634407-4634141 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References