Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5184667..5185292 Replicon chromosome
Accession NZ_LR890209
Organism Klebsiella pneumoniae isolate INF022-sc-2279895

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag JMW18_RS25265 Protein ID WP_008807903.1
Coordinates 5184667..5185050 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JMW18_RS25270 Protein ID WP_004150355.1
Coordinates 5185050..5185292 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW18_RS25250 5182033..5182935 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JMW18_RS25255 5182932..5183567 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
JMW18_RS25260 5183564..5184493 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JMW18_RS25265 5184667..5185050 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
JMW18_RS25270 5185050..5185292 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JMW18_RS25275 5185497..5186414 + 918 WP_049155024.1 alpha/beta hydrolase -
JMW18_RS25280 5186429..5187370 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
JMW18_RS25285 5187415..5187852 - 438 WP_012543288.1 D-tyrosyl-tRNA(Tyr) deacylase -
JMW18_RS25290 5187849..5188709 - 861 WP_008807907.1 virulence factor BrkB family protein -
JMW18_RS25295 5188703..5189302 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T290318 WP_008807903.1 NZ_LR890209:c5185050-5184667 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT290318 WP_004150355.1 NZ_LR890209:c5185292-5185050 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References