Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4571617..4572427 Replicon chromosome
Accession NZ_LR890204
Organism Klebsiella pneumoniae isolate INF277

Toxin (Protein)


Gene name KacT Uniprot ID -
Locus tag JMW21_RS22165 Protein ID WP_040148415.1
Coordinates 4571617..4572150 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JMW21_RS22170 Protein ID WP_002887278.1
Coordinates 4572161..4572427 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW21_RS22160 4570448..4571569 + 1122 WP_016946712.1 cupin domain-containing protein -
JMW21_RS22165 4571617..4572150 - 534 WP_040148415.1 GNAT family N-acetyltransferase Toxin
JMW21_RS22170 4572161..4572427 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JMW21_RS22175 4572530..4573963 - 1434 WP_040148413.1 Cu(+)/Ag(+) sensor histidine kinase -
JMW21_RS22180 4573953..4574636 - 684 WP_020804437.1 copper response regulator transcription factor CusR -
JMW21_RS22185 4574808..4576193 + 1386 WP_040148411.1 efflux transporter outer membrane subunit -
JMW21_RS22190 4576211..4576555 + 345 WP_040148409.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19909.78 Da        Isoelectric Point: 5.7068

>T290301 WP_040148415.1 NZ_LR890204:c4572150-4571617 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSRDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT290301 WP_002887278.1 NZ_LR890204:c4572427-4572161 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References