Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 94096..94621 Replicon plasmid 2
Accession NZ_LR890199
Organism Escherichia coli isolate MINF_1D-sc-2280456

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JMV89_RS24820 Protein ID WP_001159868.1
Coordinates 94096..94401 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JMV89_RS24825 Protein ID WP_000813634.1
Coordinates 94403..94621 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV89_RS24805 90006..91172 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
JMV89_RS24810 91760..92515 - 756 WP_024946706.1 replication initiation protein RepE -
JMV89_RS24815 93289..94095 - 807 WP_000016982.1 site-specific integrase -
JMV89_RS24820 94096..94401 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JMV89_RS24825 94403..94621 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JMV89_RS24830 95188..95700 + 513 WP_000151784.1 hypothetical protein -
JMV89_RS24835 95734..96867 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
JMV89_RS24840 97034..97807 - 774 WP_000905949.1 hypothetical protein -
JMV89_RS24845 97820..98320 - 501 WP_000528931.1 hypothetical protein -
JMV89_RS24850 98585..98815 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JMV89_RS24855 98812..99228 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / blaCTX-M-15 / sitABCD iucA / iucB / iucC / iucD / iutA 1..125802 125802


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T290269 WP_001159868.1 NZ_LR890199:c94401-94096 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT290269 WP_000813634.1 NZ_LR890199:c94621-94403 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References