Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4259973..4260549 Replicon chromosome
Accession NZ_LR890198
Organism Escherichia coli isolate MINF_1D-sc-2280456

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag JMV89_RS20400 Protein ID WP_001350372.1
Coordinates 4260262..4260549 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0G9FLJ7
Locus tag JMV89_RS20395 Protein ID WP_000063155.1
Coordinates 4259973..4260275 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV89_RS20380 4256501..4258651 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
JMV89_RS20385 4258769..4258972 + 204 WP_000467859.1 YbdD/YjiX family protein -
JMV89_RS20390 4258983..4259939 + 957 WP_001350371.1 GTPase -
JMV89_RS20395 4259973..4260275 - 303 WP_000063155.1 BrnA antitoxin family protein Antitoxin
JMV89_RS20400 4260262..4260549 - 288 WP_001350372.1 BrnT family toxin Toxin
JMV89_RS20405 4260746..4261912 + 1167 WP_000800824.1 restriction endonuclease -
JMV89_RS20410 4261976..4263595 + 1620 WP_001029741.1 type I restriction-modification system subunit M -
JMV89_RS20415 4263585..4264916 + 1332 WP_201520096.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T290259 WP_001350372.1 NZ_LR890198:c4260549-4260262 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11572.05 Da        Isoelectric Point: 9.4646

>AT290259 WP_000063155.1 NZ_LR890198:c4260275-4259973 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0G9FLJ7

References