Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2570226..2571814 Replicon chromosome
Accession NZ_LR890198
Organism Escherichia coli isolate MINF_1D-sc-2280456

Toxin (Protein)


Gene name hipA Uniprot ID A0A192ENQ5
Locus tag JMV89_RS12140 Protein ID WP_001125432.1
Coordinates 2570492..2571814 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag JMV89_RS12135 Protein ID WP_001304368.1
Coordinates 2570226..2570492 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV89_RS12120 2566594..2568045 + 1452 WP_000854639.1 tagaturonate reductase -
JMV89_RS12125 2568242..2569156 + 915 WP_001313828.1 bestrophin family inner membrane protein -
JMV89_RS12130 2569160..2569918 - 759 WP_001286543.1 trans-aconitate 2-methyltransferase -
JMV89_RS12135 2570226..2570492 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
JMV89_RS12140 2570492..2571814 + 1323 WP_001125432.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
JMV89_RS12145 2571993..2572259 - 267 WP_001527024.1 hypothetical protein -
JMV89_RS12150 2572270..2573064 - 795 WP_001527023.1 hypothetical protein -
JMV89_RS12155 2573132..2573554 - 423 WP_001527022.1 hypothetical protein -
JMV89_RS12160 2573654..2574100 - 447 WP_000527682.1 helix-turn-helix domain-containing protein -
JMV89_RS12165 2574272..2575324 + 1053 WP_000568107.1 hypothetical protein -
JMV89_RS12170 2575336..2575653 + 318 WP_000073835.1 hypothetical protein -
JMV89_RS12175 2575656..2575907 + 252 WP_000381239.1 membrane protein -
JMV89_RS12180 2575942..2576175 + 234 WP_000473544.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49297.02 Da        Isoelectric Point: 8.6972

>T290252 WP_001125432.1 NZ_LR890198:2570492-2571814 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT290252 WP_001304368.1 NZ_LR890198:2570226-2570492 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192ENQ5

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References