Detailed information of TA system TA029025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 1100100..1101821
• Accession: NC_020521
• Organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: LPE509_RS04860
• Protein ID: WP_038839099.1
• Coordinates: 1100889..1101821 (+)
• Length: 311 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A128I8L5
• Locus tag: LPE509_RS04855
• Protein ID: WP_015444253.1
• Coordinates: 1100100..1100885 (+)
• Length: 262 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPE509_RS04825	1095692..1096999	-	1308	WP_010947773.1	hypothetical protein	-
LPE509_RS04830	1097160..1097366	+	207	WP_021436844.1	hypothetical protein	-
LPE509_RS04835	1097363..1097986	-	624	WP_010947772.1	helix-turn-helix domain-containing protein	-
LPE509_RS04845	1098396..1098698	+	303	WP_010947771.1	helix-turn-helix domain-containing protein	-
LPE509_RS04850	1098611..1099615	+	1005	WP_010947770.1	site-specific integrase	-
LPE509_RS04855	1100100..1100885	+	786	WP_015444253.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LPE509_RS04860	1100889..1101821	+	933	WP_038839099.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LPE509_RS04865	1101954..1103894	-	1941	WP_038839098.1	DUF2779 domain-containing protein	-
LPE509_RS04870	1104009..1104446	-	438	WP_015444256.1	conjugal transfer protein TraM	-
LPE509_RS04875	1104446..1105171	-	726	WP_015444257.1	IncP-type DNA transfer protein TraL	-
LPE509_RS04880	1105171..1105515	-	345	WP_015444258.1	TraK family protein	-
LPE509_RS04885	1105909..1106262	+	354	WP_015444259.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1023711..1175119	151408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-264)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (97-254)

Sequences

Toxin

Protein

• Length: 311 a.a.
• Molecular weight: 35330.14 Da
• Isoelectric Point: 6.8643

>T29025 WP_038839099.1 NC_020521:1100889-1101821 [Legionella pneumophila subsp. pneumophila LPE509]
MNKQSPYYKQVLLLIRLIPLVEKETCFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPREIALANVRNALAGIAANINKI
PDLNAVLQDNKPDEMRIIVEDHSSLNRGTQIKIEVSPVARGTLLPPVELDVVASVENEFGFASMKVVSLPDLYGGKLCAA
LDRQHPRDLFDVKVLLENQKIDRELFNGFLTYLLSHKRPISEIMNPRWKDITQVFEREFKGMTAEPVTLEELTAIPGKMI
QGLKDNFTQQDYDFLVSFKRGEPEWSLACSDQIQYLPAVQWKLLNIRKMPKQKLMDSVSLLQRTMGHWLN

Nucleotide

• Length: 933 bp

>T29025 NC_020521:1100889-1101821 [Legionella pneumophila subsp. pneumophila LPE509]
ATGAATAAACAGTCACCATATTATAAACAGGTTTTGTTGCTCATTCGTTTAATTCCACTCGTCGAAAAGGAAACTTGTTT
TGCTTTAAAGGGAGGTACTGCCATTAATTTATTTGTGCGAGATTTCCCGAGGTTATCTGTGGATATAGATCTAGCGTATT
TACCCCTCGAGCCACGTGAGATTGCTTTAGCCAATGTGCGTAATGCATTAGCGGGTATTGCGGCAAATATTAATAAAATT
CCAGATTTGAATGCAGTTTTGCAAGATAACAAACCAGATGAAATGCGAATTATTGTTGAAGATCATTCTTCTCTAAATAG
GGGGACCCAAATCAAGATAGAAGTTTCACCTGTTGCGCGTGGAACTCTTTTGCCGCCAGTTGAGCTTGATGTTGTTGCTT
CGGTGGAAAATGAGTTTGGTTTTGCGTCAATGAAAGTCGTTTCTTTGCCTGATCTCTATGGTGGTAAATTATGCGCTGCT
TTAGATCGCCAGCATCCTAGAGATCTTTTTGATGTTAAGGTTTTGTTAGAAAATCAAAAGATTGACCGAGAGCTTTTTAA
TGGGTTTCTTACTTATCTTTTAAGTCACAAAAGGCCTATTTCAGAAATAATGAATCCACGATGGAAAGATATAACTCAAG
TTTTTGAACGTGAATTTAAAGGAATGACTGCTGAACCAGTTACCCTGGAAGAGCTAACAGCAATACCAGGAAAAATGATT
CAGGGTCTTAAAGATAATTTTACTCAGCAGGATTATGATTTTTTAGTCTCTTTTAAAAGGGGCGAGCCTGAGTGGTCTCT
TGCATGTAGCGATCAGATTCAATATCTCCCAGCAGTACAATGGAAGCTTTTAAATATTCGCAAAATGCCCAAACAAAAAC
TCATGGATTCAGTTAGCTTGCTACAAAGAACGATGGGACATTGGCTTAATTAG

Antitoxin

Protein

• Length: 262 a.a.
• Molecular weight: 29773.22 Da
• Isoelectric Point: 9.4457

>AT29025 WP_015444253.1 NC_020521:1100100-1100885 [Legionella pneumophila subsp. pneumophila LPE509]
MSSKINWLLNNIASGEILLQSWLSSHGITPQLARKYCQSQWLVKLGVGVYYRAGRKPRWQNAVYCLQNQSRYMIHLAGLT
SLAQQGKSHYLQFAPDSIWINVQAKTILPKWFKNFPELVISDELIPNWEIIKTSKLNAQDENDLTTIEVDGIKLKASNQE
LAAFEVLEAVPSTLSFEHAAELFQGLVNLSPRKVEHILKRSGSIQTNRLFLFLGHFYKHPWAERVNESLINLGAGKRQIV
PDGKLEKQYQITVPEQFISSD

Nucleotide

• Length: 786 bp

>AT29025 NC_020521:1100100-1100885 [Legionella pneumophila subsp. pneumophila LPE509]
ATGTCATCAAAAATAAACTGGCTTCTGAATAATATTGCCTCAGGTGAAATCTTGCTCCAGTCTTGGTTATCAAGCCATGG
AATTACTCCACAATTAGCAAGAAAATATTGTCAAAGCCAATGGTTGGTAAAATTGGGGGTAGGGGTTTATTATCGTGCAG
GGCGTAAACCTAGATGGCAAAATGCAGTTTATTGTCTTCAGAATCAGAGTCGGTACATGATTCATCTTGCTGGTTTAACC
AGTTTGGCTCAGCAGGGCAAATCGCATTACCTACAGTTTGCACCAGACTCTATTTGGATAAACGTTCAAGCAAAAACGAT
TTTACCAAAGTGGTTTAAAAATTTTCCAGAATTAGTTATTTCTGATGAACTAATACCAAATTGGGAAATCATTAAAACAT
CCAAGTTAAATGCTCAGGATGAGAATGACTTAACTACTATAGAAGTAGATGGGATTAAGTTAAAAGCAAGTAATCAAGAG
TTAGCCGCATTCGAGGTGCTGGAGGCTGTACCTTCCACTTTGTCATTTGAGCATGCAGCAGAGCTTTTTCAAGGATTGGT
AAACTTAAGTCCACGAAAAGTAGAACATATTTTAAAAAGAAGCGGATCTATTCAAACGAACCGACTTTTTTTATTTTTGG
GTCATTTCTATAAACATCCATGGGCTGAACGGGTGAATGAGTCCTTAATTAATCTTGGTGCAGGTAAGCGTCAAATAGTC
CCTGATGGAAAATTAGAAAAACAATATCAAATCACCGTTCCAGAACAATTTATAAGTAGCGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A128I8L5