Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 995188..995842 Replicon chromosome
Accession NZ_LR890198
Organism Escherichia coli isolate MINF_1D-sc-2280456

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JMV89_RS04760 Protein ID WP_000244781.1
Coordinates 995435..995842 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JMV89_RS04755 Protein ID WP_000354046.1
Coordinates 995188..995454 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV89_RS04735 991276..992709 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
JMV89_RS04740 992754..993065 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
JMV89_RS04745 993229..993888 + 660 WP_000250274.1 hemolysin III family protein -
JMV89_RS04750 993965..994945 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
JMV89_RS04755 995188..995454 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JMV89_RS04760 995435..995842 + 408 WP_000244781.1 protein YgfX Toxin
JMV89_RS04765 995882..996403 - 522 WP_001055874.1 flavodoxin FldB -
JMV89_RS04770 996515..997411 + 897 WP_000806633.1 site-specific tyrosine recombinase XerD -
JMV89_RS04775 997436..998146 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JMV89_RS04780 998152..999885 + 1734 WP_000813193.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T290244 WP_000244781.1 NZ_LR890198:995435-995842 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT290244 WP_000354046.1 NZ_LR890198:995188-995454 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References