Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2976565..2977155 Replicon chromosome
Accession NZ_LR890181
Organism Citrobacter freundii isolate MSB1_1H-sc-2280393

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag JMV71_RS14615 Protein ID WP_003836692.1
Coordinates 2976565..2976897 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag JMV71_RS14620 Protein ID WP_003836694.1
Coordinates 2976898..2977155 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV71_RS14590 2972318..2973805 + 1488 WP_003846749.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
JMV71_RS14600 2974121..2975071 - 951 WP_119174146.1 HTH-type transcriptional regulator Cbl -
JMV71_RS14605 2975173..2976090 - 918 WP_044700649.1 nitrogen assimilation transcriptional regulator NAC -
JMV71_RS14615 2976565..2976897 - 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JMV71_RS14620 2976898..2977155 - 258 WP_003836694.1 hypothetical protein Antitoxin
JMV71_RS14625 2977767..2981666 + 3900 WP_200020418.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T290195 WP_003836692.1 NZ_LR890181:c2976897-2976565 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT290195 WP_003836694.1 NZ_LR890181:c2977155-2976898 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References