Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/-
Location 3781686..3782183 Replicon chromosome
Accession NC_020518
Organism Escherichia coli str. K-12 substr. MDS42

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECMDS42_RS18315 Protein ID WP_001173343.1
Coordinates 3782010..3782183 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECMDS42_RS18310 Protein ID WP_000398619.1
Coordinates 3781686..3781982 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECMDS42_RS18285 (ECMDS42_3563) 3778417..3779136 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECMDS42_RS18290 (ECMDS42_3564) 3779133..3780764 - 1632 WP_001216477.1 sensor histidine kinase -
ECMDS42_RS18295 (ECMDS42_3565) 3780945..3781175 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
ECMDS42_RS18300 (ECMDS42_3566) 3781187..3781459 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
ECMDS42_RS18310 (ECMDS42_3567) 3781686..3781982 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECMDS42_RS18315 (ECMDS42_3568) 3782010..3782183 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECMDS42_RS18320 (ECMDS42_3569) 3782302..3783819 - 1518 WP_001295074.1 lysine--tRNA ligase -
ECMDS42_RS18325 (ECMDS42_3570) 3784056..3785513 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T29019 WP_001173343.1 NC_020518:3782010-3782183 [Escherichia coli str. K-12 substr. MDS42]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T29019 NC_020518:3782010-3782183 [Escherichia coli str. K-12 substr. MDS42]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT29019 WP_000398619.1 NC_020518:3781686-3781982 [Escherichia coli str. K-12 substr. MDS42]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT29019 NC_020518:3781686-3781982 [Escherichia coli str. K-12 substr. MDS42]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References