Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4119171..4119747 Replicon chromosome
Accession NZ_LR883050
Organism Escherichia coli isolate L1_E925

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag JL017_RS20045 Protein ID WP_001297643.1
Coordinates 4119460..4119747 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag JL017_RS20040 Protein ID WP_000063160.1
Coordinates 4119171..4119473 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL017_RS20025 4115767..4117917 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
JL017_RS20030 4117967..4118170 + 204 WP_000467859.1 YbdD/YjiX family protein -
JL017_RS20035 4118181..4119137 + 957 WP_001297640.1 GTPase -
JL017_RS20040 4119171..4119473 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
JL017_RS20045 4119460..4119747 - 288 WP_001297643.1 BrnT family toxin Toxin
JL017_RS20050 4119946..4120642 - 697 Protein_3921 IS1 family transposase -
JL017_RS20055 4120957..4122189 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
JL017_RS20060 4122230..4123510 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4119946..4120155 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T290159 WP_001297643.1 NZ_LR883050:c4119747-4119460 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT290159 WP_000063160.1 NZ_LR883050:c4119473-4119171 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References