Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/-
Location 2547074..2547728 Replicon chromosome
Accession NC_020518
Organism Escherichia coli str. K-12 substr. MDS42

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ECMDS42_RS12270 Protein ID WP_000244777.1
Coordinates 2547074..2547481 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECMDS42_RS12275 Protein ID WP_000354046.1
Coordinates 2547462..2547728 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECMDS42_RS12250 (ECMDS42_2391) 2543031..2544764 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
ECMDS42_RS12255 (ECMDS42_2392) 2544770..2545480 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECMDS42_RS12260 (ECMDS42_2393) 2545505..2546401 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECMDS42_RS12265 (ECMDS42_2394) 2546513..2547034 + 522 WP_001055874.1 flavodoxin FldB -
ECMDS42_RS12270 (ECMDS42_2395) 2547074..2547481 - 408 WP_000244777.1 protein YgfX Toxin
ECMDS42_RS12275 (ECMDS42_2396) 2547462..2547728 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECMDS42_RS12280 (ECMDS42_2397) 2547971..2548951 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ECMDS42_RS12285 (ECMDS42_2398) 2549147..2549806 - 660 WP_000250274.1 hemolysin III family protein -
ECMDS42_RS12290 (ECMDS42_2399) 2549970..2550281 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
ECMDS42_RS12295 (ECMDS42_2400) 2550326..2551759 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
ECMDS42_RS12300 (ECMDS42_2401) 2551816..2552559 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T29013 WP_000244777.1 NC_020518:c2547481-2547074 [Escherichia coli str. K-12 substr. MDS42]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T29013 NC_020518:c2547481-2547074 [Escherichia coli str. K-12 substr. MDS42]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT29013 WP_000354046.1 NC_020518:c2547728-2547462 [Escherichia coli str. K-12 substr. MDS42]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT29013 NC_020518:c2547728-2547462 [Escherichia coli str. K-12 substr. MDS42]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References