Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 297134..297934 Replicon chromosome
Accession NZ_LR883006
Organism Escherichia coli isolate L5_E1779_ETEC

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag JL014_RS01350 Protein ID WP_000342449.1
Coordinates 297407..297934 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag JL014_RS01345 Protein ID WP_001277108.1
Coordinates 297134..297400 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL014_RS01325 292791..293459 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
JL014_RS01330 293452..294510 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
JL014_RS01335 294755..295609 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
JL014_RS01340 295881..296984 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
JL014_RS01345 297134..297400 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
JL014_RS01350 297407..297934 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
JL014_RS01355 297931..298314 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
JL014_RS01360 298738..299847 + 1110 WP_001352761.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
JL014_RS01365 299895..300821 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JL014_RS01370 300818..302095 + 1278 WP_000803798.1 branched chain amino acid ABC transporter permease LivM -
JL014_RS01375 302092..302859 + 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T290092 WP_000342449.1 NZ_LR883006:297407-297934 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT290092 WP_001277108.1 NZ_LR883006:297134-297400 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References