Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3918585..3919422 Replicon chromosome
Accession NZ_LR882997
Organism Escherichia coli isolate L3_E36_ETEC

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag JL022_RS19295 Protein ID WP_000227784.1
Coordinates 3918880..3919422 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag JL022_RS19290 Protein ID WP_001297137.1
Coordinates 3918585..3918896 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL022_RS19265 3913605..3914552 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
JL022_RS19270 3914574..3916565 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JL022_RS19275 3916555..3917169 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JL022_RS19280 3917169..3917498 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JL022_RS19285 3917510..3918400 + 891 WP_000971336.1 heme o synthase -
JL022_RS19290 3918585..3918896 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
JL022_RS19295 3918880..3919422 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
JL022_RS19300 3919478..3920413 - 936 WP_001387350.1 sel1 repeat family protein -
JL022_RS19305 3920821..3922185 + 1365 WP_001000965.1 MFS transporter -
JL022_RS19310 3922313..3922804 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JL022_RS19315 3922972..3923883 + 912 WP_000705865.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T290073 WP_000227784.1 NZ_LR882997:3918880-3919422 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT290073 WP_001297137.1 NZ_LR882997:3918585-3918896 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References