Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 9604..10129 Replicon plasmid 4
Accession NZ_LR882976
Organism Escherichia coli isolate L2_E1649

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag JLZ81_RS23575 Protein ID WP_001159871.1
Coordinates 9824..10129 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JLZ81_RS23570 Protein ID WP_000813634.1
Coordinates 9604..9822 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLZ81_RS23545 5240..6190 - 951 WP_001699589.1 virulence factor VirK -
JLZ81_RS23550 6195..7283 - 1089 WP_004100285.1 putative hexosyltransferase CapU -
JLZ81_RS23555 7286..8128 - 843 WP_160371899.1 polysaccharide deacetylase family protein -
JLZ81_RS23560 8372..8902 - 531 WP_011387704.1 hypothetical protein -
JLZ81_RS23565 8902..9159 - 258 WP_000343092.1 hypothetical protein -
JLZ81_RS23570 9604..9822 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JLZ81_RS23575 9824..10129 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
JLZ81_RS23580 10130..10843 + 714 WP_000016957.1 site-specific integrase -
JLZ81_RS23585 11173..11814 + 642 WP_001705742.1 recombinase family protein -
JLZ81_RS23590 12254..12933 - 680 Protein_18 EAL domain-containing protein -
JLZ81_RS23595 13181..13884 + 704 Protein_19 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - cs3 / etpB / eltA / eltB 1..120141 120141
- flank IS/Tn - - 11173..11814 641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T290017 WP_001159871.1 NZ_LR882976:9824-10129 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT290017 WP_000813634.1 NZ_LR882976:9604-9822 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References