Detailed information of TA system TA029001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1221848..1222486
• Replicon: chromosome
• Accession: NC_020518
• Organism: Escherichia coli str. K-12 substr. MDS42

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ECMDS42_RS05930
• Protein ID: WP_000813794.1
• Coordinates: 1221848..1222024 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECMDS42_RS05935
• Protein ID: WP_001270286.1
• Coordinates: 1222070..1222486 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECMDS42_RS05910 (ECMDS42_1152)	1217467..1218681	-	1215	WP_001326689.1	BenE family transporter YdcO	-
ECMDS42_RS05915 (ECMDS42_1153)	1218734..1219270	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ECMDS42_RS05920 (ECMDS42_1154)	1219343..1221304	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECMDS42_RS05925 (ECMDS42_1155)	1221396..1221626	-	231	WP_000494244.1	YncJ family protein	-
ECMDS42_RS05930 (ECMDS42_1156)	1221848..1222024	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECMDS42_RS05935 (ECMDS42_1157)	1222070..1222486	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECMDS42_RS05940 (ECMDS42_1158)	1222565..1223971	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
ECMDS42_RS05945 (ECMDS42_1159)	1224216..1225361	+	1146	WP_000047424.1	putative ABC transporter substrate-binding protein YdcS	-
ECMDS42_RS05950 (ECMDS42_1160)	1225379..1226392	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
ECMDS42_RS05955 (ECMDS42_1161)	1226393..1227334	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T29001 WP_000813794.1 NC_020518:1221848-1222024 [Escherichia coli str. K-12 substr. MDS42]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T29001 NC_020518:1221848-1222024 [Escherichia coli str. K-12 substr. MDS42]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT29001 WP_001270286.1 NC_020518:1222070-1222486 [Escherichia coli str. K-12 substr. MDS42]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT29001 NC_020518:1222070-1222486 [Escherichia coli str. K-12 substr. MDS42]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB