Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 387833..388451 Replicon chromosome
Accession NC_020518
Organism Escherichia coli str. K-12 substr. MDS42

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ECMDS42_RS01850 Protein ID WP_001291435.1
Coordinates 387833..388051 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECMDS42_RS01855 Protein ID WP_000344800.1
Coordinates 388077..388451 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECMDS42_RS01820 (ECMDS42_0352) 383122..383694 + 573 WP_000779842.1 YbaY family lipoprotein -
ECMDS42_RS01825 (ECMDS42_0353) 383725..384114 - 390 WP_001300427.1 DNA base-flipping protein -
ECMDS42_RS01830 (ECMDS42_0355) 384415..384768 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ECMDS42_RS01835 (ECMDS42_0356) 384810..386360 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECMDS42_RS01840 (ECMDS42_0357) 386524..386994 - 471 WP_000136192.1 YlaC family protein -
ECMDS42_RS01845 (ECMDS42_0358) 387110..387661 - 552 WP_000102564.1 maltose O-acetyltransferase -
ECMDS42_RS01850 (ECMDS42_0359) 387833..388051 - 219 WP_001291435.1 HHA domain-containing protein Toxin
ECMDS42_RS01855 (ECMDS42_0360) 388077..388451 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECMDS42_RS01860 (ECMDS42_0361) 388997..392146 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
ECMDS42_RS01865 (ECMDS42_0362) 392169..393362 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T28998 WP_001291435.1 NC_020518:c388051-387833 [Escherichia coli str. K-12 substr. MDS42]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T28998 NC_020518:c388051-387833 [Escherichia coli str. K-12 substr. MDS42]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT28998 WP_000344800.1 NC_020518:c388451-388077 [Escherichia coli str. K-12 substr. MDS42]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT28998 NC_020518:c388451-388077 [Escherichia coli str. K-12 substr. MDS42]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References