Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1143815..1144451 Replicon chromosome
Accession NC_020517
Organism Bifidobacterium breve UCC2003

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag BBR_RS15030 Protein ID WP_003829234.1
Coordinates 1143815..1144171 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag BBR_RS15035 Protein ID WP_012577921.1
Coordinates 1144158..1144451 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBR_RS15000 1139003..1139914 + 912 WP_015438746.1 ABC transporter ATP-binding protein -
BBR_RS15005 1140111..1140737 + 627 WP_003829226.1 30S ribosomal protein S4 -
BBR_RS15010 1141074..1141856 - 783 WP_015438747.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
BBR_RS15015 1141999..1142484 - 486 WP_003829229.1 cytidine deaminase -
BBR_RS15020 1142575..1142931 + 357 WP_003833231.1 SdpI family protein -
BBR_RS15025 1143036..1143638 + 603 WP_003829233.1 transglutaminase family protein -
BBR_RS15030 1143815..1144171 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BBR_RS15035 1144158..1144451 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BBR_RS15040 1144602..1144766 - 165 Protein_940 IS30 family transposase -
BBR_RS15045 1144866..1145540 + 675 WP_015438748.1 histidine phosphatase family protein -
BBR_RS15050 1145623..1146027 + 405 WP_003829238.1 DUF948 domain-containing protein -
BBR_RS15055 1146027..1146263 + 237 WP_003829239.1 hypothetical protein -
BBR_RS15060 1146359..1149037 + 2679 WP_003829240.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T28994 WP_003829234.1 NC_020517:c1144171-1143815 [Bifidobacterium breve UCC2003]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T28994 NC_020517:c1144171-1143815 [Bifidobacterium breve UCC2003]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGTACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT28994 WP_012577921.1 NC_020517:c1144451-1144158 [Bifidobacterium breve UCC2003]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT28994 NC_020517:c1144451-1144158 [Bifidobacterium breve UCC2003]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References