Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1073438..1073993 Replicon chromosome
Accession NC_020517
Organism Bifidobacterium breve UCC2003

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag BBR_RS14700 Protein ID WP_003829109.1
Coordinates 1073438..1073761 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag BBR_RS14705 Protein ID WP_003829112.1
Coordinates 1073748..1073993 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBR_RS14675 1068466..1068764 - 299 Protein_866 IS256 family transposase -
BBR_RS14680 1069234..1070499 - 1266 WP_015438706.1 Fic family protein -
BBR_RS14685 1071041..1071406 - 366 WP_025299744.1 YccF domain-containing protein -
BBR_RS14690 1071644..1072513 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
BBR_RS20845 1072607..1073221 + 615 WP_003829107.1 cupin domain-containing protein -
BBR_RS14700 1073438..1073761 - 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BBR_RS14705 1073748..1073993 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BBR_RS14710 1074165..1075421 + 1257 WP_015438708.1 HipA domain-containing protein -
BBR_RS14715 1075687..1075865 + 179 Protein_874 XRE family transcriptional regulator -
BBR_RS14720 1076049..1077320 - 1272 WP_003829121.1 MFS transporter -
BBR_RS21315 1077477..1077686 - 210 Protein_876 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1063206..1080728 17522


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T28993 WP_003829109.1 NC_020517:c1073761-1073438 [Bifidobacterium breve UCC2003]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T28993 NC_020517:c1073761-1073438 [Bifidobacterium breve UCC2003]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT28993 WP_003829112.1 NC_020517:c1073993-1073748 [Bifidobacterium breve UCC2003]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT28993 NC_020517:c1073993-1073748 [Bifidobacterium breve UCC2003]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References